منابع مشابه
Enzymes of plasma membranes of liver.
The aim of the work was to provide tentative criteria for distinguishing between authentic and contaminating activities of plasma membrane preparations from rat liver. To this end, the specific activities and extractabilities were studied of eight activities found in the membrane preparations and in liver microsomes. Liver microsomes were examined since contaminants of the plasma membrane prepa...
متن کاملISOLATION OF RAT LIVER PLASMA MEMBRANES Use of Nucleotide Pyrophosphatase and Phosphodiesterase I as Marker Enzymes
Nucleotide pyrophosphatase and phosphodiesterase I of rat liver have been found to be localized primarily in cell particulates highly enriched with respect to the most commonly accepted plasma membrane marker, 5'-nucleotidase, and therefore should themselves be assigned a plasma membrane localization. The observation that plasma membranes sediment in isotonic sucrose with both nuclear and micro...
متن کاملEnzymes linked to phosphatidylinositol in plasma membranes.
Treatment of tissues, isolated cells, and cell homogenates and fractions with a phosphatidylinositol-specific phospholipase C has demonstrated a selective release of several enzymes from plasma membranes. The purified phospholipase has no detectable action on mammalian phospholipids other than phosphatidylinositol and although this phospholipid appears to form only a very small proportion of th...
متن کاملPlasma Membranes of the Rat Liver
A method is described for the rapid isolation of a plasma membrane fraction containing a high concentration of intact bile canaliculi from the rat liver. Isolated bile canaliculi retain most of the ultrastructural features exhibited in the intact liver cell. The final fraction contains 5'-nucleotidase activity at approximately the same concentration as that in previous preparations of plasma me...
متن کاملIsolation of Rat Liver Plasma Membranes
Nucleotide pyrophosphatase and phosphodiesterase I of rat liver have been found to be localized primarily in cell particulates highly enriched with respect to the most commonly accepted plasma membrane marker, 5'-nucleotidase, and therefore should themselves be assigned a plasma membrane localization. The observation that plasma membranes sediment in isotonic sucrose with both nuclear and micro...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1967
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)96068-0